The chromatogram showed some additional peaks after 2 h in bench top conditions and after 12 h in refrigeration conditions. It was concluded that the solution was stable for 1 h at bench top conditions and 6 h at refrigeration conditions [Figure 6]. Figure 6 Solution stability for montelukast at refrigrator conditions after 12 h. Additional selleck catalog peaks obtained at 7.2 min and 23.7 min CONCLUSIONS It is a well known that the validation procedure is an integral part of the analytical method development. Therefore, the developed method was validated according to the ICH guidelines Q2 (R1). Based on the results, it can be concluded that there is no other co-eluting peak with the main peaks and that the method is specific for estimation of montelukast sodium and doxofylline.
The proposed method has a linear response in the stated range and is accurate and precise. To our knowledge, the developed HPLC method is the first reported method for simultaneous determination of montelukast sodium and doxofylline from their combination drug product with very less retention time (3.408 min for doxofylline and 5.506 min for montelukast sodium) using a C8 column. Then, the stability study indicated that the standard stock solution was stable up to 6 h in the refrigrator. Therefore during the analysis, the standard and sample solutions should be kept in the refrigrator and used with in 6 h to get the better results. Taken together, these results clearly showed that this method can be used for routine analysis of montelukast sodium and doxofylline in their combined dosage form.
The developed method can also be conveniently adopted for dissolution testing of tablets containing montelukast sodium and doxofylline. Footnotes Source of Support: Nil Conflict of Interest: None declared.
Nabumetone (NBM) is frequently prescribed as a nonsteroidal anti-inflammatory drug for the symptomatic treatment of rheumatic and inflammatory conditions.[1,2] NBM is chemically known as 4-(6-methoxy-2-naphthyl)-butan-2-one. It is official in United States Pharmacopoeia and British Pharmacopoeia. Paracetamol (PRCM) [Figure 1] is official in Indian Pharmacopoeia, and it is chemically N-(4-hydroxyphenyl) acetamide and is an analgesic and antipyretic drug. The derivative spectrophotometric method is one of the advanced modern spectrophotometric techniques that offer a useful means for extracting both qualitative and quantitative information from the spectra composed of overlapped bands.
It is based on using the first- or higher-order derivatives of absorbance with respect to wavelength from parent zero-order ones. Because derivatization can lead to the separation of unresolved signals and reduction of spectral background interferences, this technique permits the quantification of Entinostat one analyte in the presence of others without initial separation or purification.