7%), ‘water’ (4.3%), ‘sediment’ (4.3%), ‘sea’ (3.5%) and ‘coastal’ (2.6%) (115 hits in total). Environmental samples which yielded Trichostatin A 58880-19-6 hits of a higher score than the highest scoring species were not found. The environmental samples database (env_nt) contains the marine metagenome clone ctg_1101667042524 (“type”:”entrez-nucleotide”,”attrs”:”text”:”AACY022635173″,”term_id”:”131062951″,”term_text”:”AACY022635173″AACY022635173) isolated from Sargasso Sea near Bermuda, sharing 92% identity with IC166T [7] (as of January 2011). Figure 1 shows the phylogenetic neighborhood of C. algicola IC166T in a 16S rRNA based tree. The sequences of the five 16S rRNA gene copies in the genome differ from each other by up to two nucleotides, and differ by up to 14 nucleotides from the previously published 16S rRNA sequence (“type”:”entrez-nucleotide”,”attrs”:”text”:”AF001366″,”term_id”:”2072540″,”term_text”:”AF001366″AF001366), which contains nine ambiguous base calls.

Figure 1 Phylogenetic tree highlighting the position of C. algicola IC166T relative to the other type strains within the family Flavobacteriaceae. The tree was inferred from 1,458 aligned characters [8,9] of the 16S rRNA gene sequence under the maximum likelihood … The cells of C. algicola are generally rod-shaped with rounded or tapered ends with cell lengths and widths ranging from 1.5 to 4 and 0.4 to 0.5 ��m, respectively (Figure 2 and Table 1). C. algicola is motile by gliding [1]. Colonies on marine 2216 agar have yellow-orange pigmentation and a compact center, with a spreading edge possessing lighter pigmentation.

Their consistency is slimy and they are slightly sunken into the agar [1]. Flexirubin pigments are not formed. C. algicola grows between 0.5 and 10% NaCl, with the best growth in the presence of about 2% NaCl. The temperature range for growth is between -2��C and 28��C, with an optimum between 15-20��C on solid media and at about 20-25��C in liquid media [1]. The optimal pH for growth is about 7.5 [1]. Figure 2 Scanning electron micrograph of C. algicola IC166T Table 1 Classification and general features of C. algicola IC166T according to the MIGS recommendations [13]. The organism is strictly aerobic and chemoorganotrophic [1]. C. algicola can hydrolyze agar, starch, gelatine, carboxymethylcellulose (CMC), casein, Tween 80, tributyrin and L-tyrosine, but not urate, xanthine or dextran, when grown in presence of 1% L-tyrosine a reddish-brown diffusible pigment is formed [1].

Nitrate reduction is positive, whereas denitrification, H2S production and indole production are negative [1,18]. Acid is formed oxidatively from D-galactose, D-glucose, D-fructose, sucrose, cellobiose, lactose and mannitol. Strain IC166T is sensitive to ampicillin, streptomycin and carbenicillin and shows resistance to tetracycline [3]. Chemotaxonomy The fatty acid Anacetrapib profile of seven Antarctic strains, including strain IC166T, was analyzed by Bowman in 2000 [1].