So, it was revealed that the peaks obtained
from drug-polymer matrix not significantly shifted to lower or higher intensity than metformin HCl peak. It means there was not chemical interaction between metformin HCl and ethylcellulose polymer. The X-ray diffraction graph of same are illustrate in Fig. 3. Percentage crystallinity of metformin HCl was 98.6% and gives characteristic intense peaks at 2θ of 17.67 °C, 22.36 °C, 23.26 °C, 24.63 °C, 26.43 °C, 27.23 °C, 28.28 °C, 29.53 °C. EC45, EC100, EC300 coated nanoparticles were 45.9%, 42.4% and 36.9% crystallinity respectively and amorphous in nature. Amorphous character of nanoparticles may be due to ethylcellulose overlapped on metformin HCl which shows the drug is dispersed at molecular level in polymer matrix or intervention of EC
find more molecules arrangement in metformin molecules during solidification or precipitation can cause amorphous nature. Although there were good encapsulation efficiency in all three polymers at different ratios means not necessary to sustained metformin capably. This was clarified in dissolution test of all batches (Fig. 4). As drug-polymer ratio increased the sustainability of formulations also increased. 1:9 ratio was more sustained than other two ratios. EC45, EC100 and EC300 released 64.56 ± 0.29, 58.75 ± 0.12 selleck compound and 44.83 ± 0.09 percent drug respectively within 12 h from more sustained 1:9 ratio formulations. So, EC300 was more sustained than EC45 and EC100. Burst release was observed in low drug-polymer ratios of EC45 and EC100 nanoparticles. After released surface drug in first hour, near about 20–25% drug was released from next to 12 h. As shown in figure this release rate was constant for all nanoparticles formulations. At lower drug-polymer ratios the available polymer concentration may be insufficient to coat all amount of drug, therefore some drug might moved toward the interface of internal and external phase due to surfactant susceptibility migrate toward the surface of ethylcellulose nanoparticles.
During evaporation of organic solvent the drug available on surface of globules get precipitate first and Oxalosuccinic acid stable over there. This drug at the surface released within first hour of dissolution and confers burst release effect.8 and 14 Remaining drug in the core of particle might strongly matrixes with polymer which released slowly over maximum period. Increased in drug-polymer ratios decreased the first high release of metformin HCl and also provide strong binding between drug and polymer. From dissolution study it was also revealed that more viscosity grade ethylcellulose sustained drug efficiently than low viscosity grade ethylcellulose polymer. The order of sustainability of ethylcellulose polymer was EC300 > EC100 > EC45.