1038/labinvest.2009.82; published online 3 August 2009″
“One difficulty in studying dengue virus (DENV) is the lack of an experimental model that reproduces the human disease. In a previous work, we have shown that BALB/c mice intraperitoneally inoculated with a DENV-2 isolate presented viremia and mild focal areas
of liver injuries. In this study, mice were inoculated by the intravenous route and presented extensive damage areas in the liver tissue, which were evaluated by histopathological and ultrastructural analysis. Hepatic injury was noted mainly around the central vein and portal tracts. Damages consist of hepatocyte injury, including steatosis, swelling and necrosis. Further, erythrophagocytosis, selleck compound intercellular edema and vascular damages were evident, including hemorrhage, which is characteristic of the dengue-induced hepatitis in human liver. Hepatic lesions were already noted 2 days post infection (p.i.), although effects were more extensive after the seventh day p.i. An increase in alanine aminotransferase and aspartate aminotransferase serum levels was detected 7 and 14 days p.i., respectively, and had correlation Selinexor to hepatic lesions. Alterations caused by the DENV infection were self-limiting, with a remarkable reduction of all liver damages 49 days p.i. Virus antigens were detected in hepatocytes, Kupffer cells and vascular endothelium,
suggesting virus replication in these cells. In situ hybridization, using a probe that anneals in the virus negative RNA strand, showed positive reaction in hepatocytes and vascular endothelium cells of infected mice, thus confirming virus replication in such cells. In general, ASK1 results revealed
that this mouse model reproduces some histopathological effects observed in humans and supports previous findings indicating virus replication in the hepatic tissue. Laboratory Investigation (2009) 89, 1140-1151; doi:10.1038/labinvest.2009.83; published online 31 August 2009″
“This study evaluates the intra- and inter-subject variability of digit maps in area 3b of anesthetized squirrel monkeys. Maps were collected using high field blood oxygenation level dependent (BOLD) functional magnetic resonance imaging (fMRI). BOLD responses to individual digit stimulations were mapped and their response properties (location, area of activation, % signal change, time to peak response) were compared within and across imaging sessions separated by up to 20 months. During single digit stimulation using a block design, the spatiotemporal response of the BOLD signal for individual runs within and across sessions and animals was well conserved, with a time to peak BOLD response of 20 +/- 4 s. The variability in the center of BOLD activation in area 3b was 0.41 +/- 0.24 mm (mean +/- SD) across individual 5-7 min runs within a scanning session and 0.55 +/- 0.15 mm across sessions. The average signal change across all animals, runs and sessions was 0.62 +/- 0.38%, and varied 32% within and 40% across sessions.