The reduced-expression
of E-CAD and over-expression of MMP-7 may be important promoting factors in invasion and metastasis of colorectal carcinoma. They may be valuable indicators for diagnosing in early colorectal carcinoma, selecting therapy and assessing prognosis. Key Word(s): 1. Colorectal carcinoma; 2. Immunohistochemistry; 3. E-cadherin; 4. Matrix m-7; Presenting LGK-974 ic50 Author: CHENYING YING Corresponding Author: CHENYING YING Affiliations: First Affiliated Hospital of Harbin Medical University Objective: To investigate the gene polymorphisms of interferon-γ(IFN-γin patients with ulcerative colitis(UC), as well as the relationship of UC pathogenesy and gene polymorphism. Methods: The cytokine genotypes of IFN-γfrom UC(n = 56) and normal persons(n = 44) were detected by Sequence- Specific Primers polymerase chain reaction (PCR-SSP). And the serum levels of cytokines were assayed by ELISA. Results: The genotype frequency and allelic frequency of IFN-γ+874 in UC patients had no significant difference compared with that in normal control cases (P > 0.05). Each genotype frequency of IFN-γ+874 had no significant difference among UC with three regionals (P > 0.05). The level of serum IFN-γin active UC was much higher
than that in catabolic UC and control group (P < 0.05). There were no significantly difference of IFN-γamong different genotypes in UC groups. (P > 0.05). Conclusion: The polymorphisms of IFN-γ+874 may have no influence on RNA Synthesis inhibitor the susceptibility to UC. Genotypes may be the determinants click here of their corresponding serum levels in healthy adult people, however, the serum levels in UC patients were also influenced by other
factors simultaneously. Key Word(s): 1. interferon-γ; 2. Ulcerative colitis; 3. Gene polymorphisms; Presenting Author: JIANG MIAO Corresponding Author: JIANG MIAO Affiliations: The First Affiliated Hospital of Harbin Medical University Objective: To study the apoptosis effect of Arsenic trioxideon on human gastric and colorectal adenocarcinoma cells and mechanisms and the relation between this apoptosis and expression of p53 and bcl -2. Methods: Intravenous administration of Arsenic trioxideon at 10 mg/ day for 3 days were carried out preoperatively. The expression of p53, bcl-2 and apoptosis induced by arsenic trioxide were examined by immunohistochemistry method and TUNEL. Results: Arsenic trioxide induced decrease of the expression of bcl -2 and increase of the expression of apoptosis in gastric and colorectal cancer cells. The expression of p53 was not changed by As2O3. Conclusion: Preoperatively intravenous chemotherapy with Arsenic trioxide can induce apoptosis and inhibite proliferation effectively in gastric and colorectal cancer. Arsenic trioxide induce the apoptosis of gastric and colorectal cancer cells through accommodating the expression of cancer associated genes. Key Word(s): 1. Arsenic trioxide; 2.