The genus was named in honor of Leslie Turner, an English microbiologist who made definitive contributions to the knowledge of leptospirosis [1]. However, as the generic name is also in use in botany and http://www.selleckchem.com/products/Abiraterone.html zoology, this name was rendered illegitimate and invalidate, but was used in the literature [6,7]. The first 16S rRNA gene-based study (Genbank accession number “type”:”entrez-nucleotide”,”attrs”:”text”:”Z21636″,”term_id”:”433587″,”term_text”:”Z21636″Z21636), performed on Leptospira parva incertae sedis, confirmed the isolated position of L. parva among Leptonema and Leptospira species [8], a finding later supported by Morey et al. [9]. The reclassification of L. parva as Turneriella parva com. nov. was published by Levett et al.

[1], reconfirming the separate position of the type strain [10] and an additional strain (S-308-81, ATCC BAA-1112) from the uterus of a sow from all other leptospiras on the basis of DNA-DNA hybridization and 16S rRNA gene sequence analysis (Genbank accession number “type”:”entrez-nucleotide”,”attrs”:”text”:”AY293856″,”term_id”:”31580622″,”term_text”:”AY293856″AY293856). The strain was selected for genome sequencing because of its deep branching point within the Leptospiraceae lineage. Here we present a summary classification and a set of features for T. parva HT together with the description of the complete genomic sequencing and annotation. Classification and features 16S rRNA gene sequence analysis A representative genomic 16S rDNA sequence of T. parva HT was compared using NCBI BLAST [11,12] under default settings (e.g.

, considering only the high-scoring segment pairs (HSPs) from the best 250 hits) with the most recent release of the Greengenes database [13] and the relative frequencies of taxa and keywords (reduced to their stem [14]) were determined, weighted by BLAST scores. The most frequently occurring genera were Geobacter (48.7%), Leptospira (19.2%), Pelobacter (13.4%), Spirochaeta (8.1%) and Turneriella (6.4%) (56 hits in total). Regarding the single hit to sequences from members of the species, the average identity within HSPs was 95.8%, whereas the average coverage by HSPs was 89.8%. Among all other species, the one yielding the highest score was Leptonema illini (“type”:”entrez-nucleotide”,”attrs”:”text”:”AY714984″,”term_id”:”51950701″,”term_text”:”AY714984″AY714984), which corresponded to an identity of 85.

7% and an HSP coverage of 62.6%. (Note that the Greengenes database uses the INSDC (= EMBL/NCBI/DDBJ) annotation, which is not an authoritative source for nomenclature or classification.) The highest-scoring environmental sequence was “type”:”entrez-nucleotide”,”attrs”:”text”:”DQ017943″,”term_id”:”64330466″,”term_text”:”DQ017943″DQ017943 (Greengenes short name ‘Cntrl Erpn Rnnng Wtrs Exmnd Cilengitide TGGE and uplnd strm cln S-BQ2 83′), which showed an identity of 95.