The genus was named in honor of Leslie Turner, an English microbi

The genus was named in honor of Leslie Turner, an English microbiologist who made definitive contributions to the knowledge of leptospirosis [1]. However, as the generic name is also in use in botany and http://www.selleckchem.com/products/Abiraterone.html zoology, this name was rendered illegitimate and invalidate, but was used in the literature [6,7]. The first 16S rRNA gene-based study (Genbank accession number “type”:”entrez-nucleotide”,”attrs”:”text”:”Z21636″,”term_id”:”433587″,”term_text”:”Z21636″Z21636), performed on Leptospira parva incertae sedis, confirmed the isolated position of L. parva among Leptonema and Leptospira species [8], a finding later supported by Morey et al. [9]. The reclassification of L. parva as Turneriella parva com. nov. was published by Levett et al.

[1], reconfirming the separate position of the type strain [10] and an additional strain (S-308-81, ATCC BAA-1112) from the uterus of a sow from all other leptospiras on the basis of DNA-DNA hybridization and 16S rRNA gene sequence analysis (Genbank accession number “type”:”entrez-nucleotide”,”attrs”:”text”:”AY293856″,”term_id”:”31580622″,”term_text”:”AY293856″AY293856). The strain was selected for genome sequencing because of its deep branching point within the Leptospiraceae lineage. Here we present a summary classification and a set of features for T. parva HT together with the description of the complete genomic sequencing and annotation. Classification and features 16S rRNA gene sequence analysis A representative genomic 16S rDNA sequence of T. parva HT was compared using NCBI BLAST [11,12] under default settings (e.g.

, considering only the high-scoring segment pairs (HSPs) from the best 250 hits) with the most recent release of the Greengenes database [13] and the relative frequencies of taxa and keywords (reduced to their stem [14]) were determined, weighted by BLAST scores. The most frequently occurring genera were Geobacter (48.7%), Leptospira (19.2%), Pelobacter (13.4%), Spirochaeta (8.1%) and Turneriella (6.4%) (56 hits in total). Regarding the single hit to sequences from members of the species, the average identity within HSPs was 95.8%, whereas the average coverage by HSPs was 89.8%. Among all other species, the one yielding the highest score was Leptonema illini (“type”:”entrez-nucleotide”,”attrs”:”text”:”AY714984″,”term_id”:”51950701″,”term_text”:”AY714984″AY714984), which corresponded to an identity of 85.

7% and an HSP coverage of 62.6%. (Note that the Greengenes database uses the INSDC (= EMBL/NCBI/DDBJ) annotation, which is not an authoritative source for nomenclature or classification.) The highest-scoring environmental sequence was “type”:”entrez-nucleotide”,”attrs”:”text”:”DQ017943″,”term_id”:”64330466″,”term_text”:”DQ017943″DQ017943 (Greengenes short name ‘Cntrl Erpn Rnnng Wtrs Exmnd Cilengitide TGGE and uplnd strm cln S-BQ2 83′), which showed an identity of 95.

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