Luciferase signals in the abdominal area of LPS treated mice were quantified making use of the Living Image soft ware to create the data shown in Figure 1B. In the peak of induction two to 4 hours soon after injection, the luciferase sig nals have been increased six to ten fold by LPS as compared with basal luciferase signal at T 0 hour. At 24 hours, the luci ferase signal was nevertheless 2 to 3 fold higher than basal levels. IB expression is induced in multiple tissues following LPS remedy Table 1 displays the luciferase activity in selected organs in IB luc mice. In untreated mice, ex vivo luciferase activity was detected in each of the dissected organs of both sexes. The pattern of luciferase expression on the male tissues was similar to that on the female tissues.
The NSC 74859 price luciferase activity was the highest in liver, spleen and lung, lowest in heart, and intermediate in intestine, kidney and brain. In LPS treated mice, each of the examined organs showed a considerable induction of the luciferase activity. Liver, spleen, lung and intestine showed substantially higher luciferase expres sion than that in kidney, heart and brain. As calculated in the mean from the control mice, LPS therapy triggered 19 to 23 fold luciferase induction inside the liver, 19 to 28 fold in the spleen, 8 fold in the lung, 19 to 52 fold inside the intestine, six to 11 fold in the kidney, 54 to 63 fold in the heart, five to 7 fold in the brain. We further attempted to establish a correlation among luciferase activity and IB mRNA expression. Within the liver tissue of un treated mice, IB mRNA expression was detectable.
Following LPS remedy, an induction of IB mRNA expression was observed, which corre lated together with the raise of luciferase activity inside the liver. Bortezomib inhibited LPS induced IB expression Applying the IB luc model, we tested the impact of borte zomib on LPS induced IB expression in vivo. As shown in Figure 2A, pre Baricitinib remedy of your IB luc mice with bort ezomib significantly inhibited LPS induced luciferase expression inside the complete body, in particular in liver and intes tine exactly where the luciferase signal was very induced. Quan tification from the luciferase signal showed that inhibition of luciferase activity by bortezomib was important at all of the time points in both male and female mice. In the peak of induction at two 4 hours, bortezomib inhib ited 70 80% of LPS induced luciferase activity inside the abdominal area. Bortezomib inhibited LPS induced IB expression in all the organs except the brain We examined the effect of bortezomib on LPS induced IB expression in chosen organs. In com parison for the LPS treated mice, mice pre treated with bortezomib showed considerable inhibition of luciferase induction in all organs examined except the brain.