Key Word(s): 1. Colorectal cancer; 2. Colonoscopy; 3. Early stage tumor; 4. Rightward shift; Presenting Author: SUJUN HUANG Additional Authors: BINWEN WU, DONGFENG LI, XIAONAN ZHANG, GANG DENG, KAIJUN ZHANG Corresponding Author: SUJUN HUANG

Affiliations: Guangdong General Hospital Objective: Astrocyte elevated gene-1 (AEG-1), upregulated in various types of malignancies including colorectal cancer, has been reported to be associated with the carcinogenesis of human cancer. However, the functional selleck products significance of AEG-1 in human colon cancer remains unclear. The aim of this study was to investigate whether AEG-1 could serve as a potential therapeutic target of human colon cancer. Methods: We document that AEG-1 expression is high in human colon cancer cell lines HCT116 but relatively low in SW1116 by Western Blot. RNA interference was used to reduce AEG-1 expression in HCT116 and their phenotypic changes were analyzed. Meanwhile, the expression of AEG-1 in SW1116 were enhanced to analyzed their phenotypic changes. Moreover, MTT assay was used to detect the chemo-sensitivity of cells. Results: Knockdown of AEG-1 expression in human colon cancer

cells could significantly inhibit colon cancer cell proliferation and colony formation. The specific downregulation induced cell arrest in G0/G1 phase of cell cycle. Conversely, upregulation of PLX 4720 AEG-1 could significantly enhance cell proliferation and migration. Moreover, AEG-1 directly contributed to chemoresistance to 5-fluorouracil (5-Fu) in HCT116 and SW1116-AEG-1. Conclusion: Targeted inhibition of AEG-1 can lead to shutdown of key elemental characteristics of colon cancer cells and increases the sensitivity of colon cancer cells to 5-fluorouracil, which may become an potential effective therapeutic strategy for colon cancer. Key Word(s): 1. AEG-1; 2. Colon cancer; 3. chemoresistance; Presenting

Author: MARIA FATIMABUSTILLO SABATEN Additional Authors: SOPHIAMEJIA ZAMORA, JOHN PAUL MALENAB Corresponding Author: MARIA FATIMABUSTILLO SABATEN Affiliations: Manila Doctors Hospital Objective: Intussusceptions were 上海皓元医药股份有限公司 first described in 1674 by Barbette of Amsterdam.1 Their occurrence in adults is rare, accounting for less than 5% of all cases of intussusceptions and almost 1%–5% of bowel obstruction.1 Lipomas are relatively uncommon, slow-growing, benign, non-epithelial, fatty tumors that can be found throughout the gastrointestinal tract, although most frequently seen in the colon.2,3 They were first described by Baurer in 1757.4 The reported incidence of lipomas in the large intestine ranges from 0.2%–4.4%.4 The clinical presentation is very nonspecific and runs a silent clinical course which makes this a difficult condition to diagnose.2. Methods: We present a case of a 72 year old female with an intussusception caused by a colonic lipoma presenting with an eight months history of recurrent left lower quadrant pain with intermittent diarrhea.