In our examine, we investigated the chemosensitivity of CpG ODN in HepG2 human hepatoma cells, the HepG2 cells were cultured in vitro, plus the cell proliferation, apop tosis, cell cycle as well as antiapoptotic components were examined. read more here The feasible molecular mechanisms have been investigated. Results CpG ODN in bination with 5 FU TWS119 decreases cell viability in HepG2 cells Prior research showed that TLR9 is expressed in hu guy lung cancer A549 cells and human hepatoma HepG2 cells TLR9 expression and function in BEL 7402 cells, belong to the human hepatoma cell lines, aren’t reported. The expression of functional lively TLR9 in human malignant tumors could possibly impact therapy ap proaches implementing CpG ODN. To evaluate the cytotoxicity of CpG ODN on HepG2 cells, BEL 7402 cells and A549 cells, cells had been incubated with 5 gradient concentra tion ranging from 0. 25 to 25 uM for 48 h.
The viability was determined implementing CellTiter 96Aqueous One particular Solu tion Cell Proliferation Assay The outcomes showed that CpG ODN appreciably decreased the viability of HepG2 cells in the dose dependent method. Having said that, CpG ODN had no result around the viability of BEL 7402 cells and A549 cells These findings indi cated that HepG2 cells, but not BEL 7402 cells and A549 cells, are delicate to CpG ODN. Taking into ac count the substantial inhibition of CpG ODN on HepG2 cells, and it was picked and utilized in the subsequent experiments. could encourage cell proliferation and survival in human hepatocellular carcinomas. Herein, we pared the cytotoxicity of non CpG ODN,CpG ODN and ODN M362 toward HepG2 cells, cells have been handled with non CpG ODN, CpG ODN or ODN M362 at five concentra tion ranging from 0. 25 to 25 uM for the indicated time. The consequence showed that CpG ODN considerably reduced the viability of HepG2 cells in time and dose dependent method However, non CpG ODN and ODN M362 didnt have direct cytotoxicity toward HepG2 cells The outcomes showed that CpG ODN, but not ODN M362 and non CpG ODN, can dir ectly mediate cytotoxicity towards HepG2 cells.