In API 50CH, the strain had the following characteristics: positive for acid production from d-arabinose, l-arabinose, d-xylose, d-galactose, d-glucose, d-fructose, d-mannose, aesculin,

d-cellobiose, d-maltose, d-lactose, d-melibiose, sucrose, d-trehalose, d-raffinose and starch; weakly positive for acid production from n-acetyl-glucosamine and amygdalin; and negative for acid production from glycerol, erythritol, d-ribose, l-xylose, d-adonitol, methyl-β,d-xylopyranoside, l-sorbose, l-rhamnose, dulcitol, inositol, d-mannitol, d-sorbitol, inulin, d-melezitose, xylitol, gentiobiose, d-turanose, d-lyxose, d-tagatose, d-fucose, l-fucose, d-arabitol, l-arabitol, potassium gluconate, potassium 2-keto-gluconate and potassium 5-keto-gluconate. The strain is sensitive click here to tetracycline and clindamycin, but resistant to ampicillin, amikacin, ceftriaxone, gentamicin, kanamycin,

neomycin, penicillin, streptomycin and vancomycin. The only major isoprenoid quinone is MK-7. The predominant fatty acid is summed feature 3 (C16:1ω7c and/or iso-C15:0 2OH). The genomic DNA G+C content is 42.6 mol%. The type strain is DR-f4T (=KACC 14556T=JCM 16601T), isolated from the rhizosphere of P. grandiflorum collected at Chungcheongnam-Do, Korea. We thank Dr Bernhard Schink for his advice on the Latin naming of the organism. This work was supported by the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government (MEST) Casein kinase 1 (no. R01-2007-000-21120-0), grant NMC0300938 and grant from the KRIBB Research Initiative Program. The GenBank/EMBL/DDBJ see more accession number for the 16S rRNA gene sequence of the strain DR-f4T is GU139697. Fig. S1. Electron micrograph of Mucilaginibacter dorajii DR-f4T grown on R2A plate at 25°C for 3 days. Please note: Wiley-Blackwell is not responsible for the content or functionality of any supporting materials supplied by the authors. Any queries (other than missing material) should

be directed to the corresponding author for the article. “
“Xanthomonas citri ssp. citri (Xac) is the causal agent of citrus canker, an economically important disease that affects citrus worldwide. To initiate the characterization of essential biological processes of Xac, we constructed integrative plasmids for the ectopic expression of green fluorescent protein (GFP)-labeled proteins within this bacterium. Here, we show that the disruption of the α-amylase gene (amy), the site of plasmid integration into the bacterial chromosome, does not alter its pathogenesis while abolishing completely the ability of Xac to degrade starch. Furthermore, our GFP expression system was used to characterize ORF XAC3408, a hypothetical protein encoded by Xac that shares significant homology to the FtsZ-stabilizing factor ZapA from Bacillus subtilis (ZapABsu).