From four independent experiments in the NCI-60 screen, the 50% growth inhibitory concentration (GI50) for the 6 leukemia cell lines ranged from 40 nM -630 nM,
and the GI50 for NCI-H522 was 79 nM, which was 10-fold more sensitive than the average response for the whole Talazoparib cell line panel (762 nM) (data available at: http://dtp.nci.nih.gov/ for NSC 680410). Transcriptional profiling of NCI-H522 in response to 1 μM {Selleck Anti-infection Compound Library|Selleck Antiinfection Compound Library|Selleck Anti-infection Compound Library|Selleck Antiinfection Compound Library|Selleckchem Anti-infection Compound Library|Selleckchem Antiinfection Compound Library|Selleckchem Anti-infection Compound Library|Selleckchem Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|buy Anti-infection Compound Library|Anti-infection Compound Library ic50|Anti-infection Compound Library price|Anti-infection Compound Library cost|Anti-infection Compound Library solubility dmso|Anti-infection Compound Library purchase|Anti-infection Compound Library manufacturer|Anti-infection Compound Library research buy|Anti-infection Compound Library order|Anti-infection Compound Library mouse|Anti-infection Compound Library chemical structure|Anti-infection Compound Library mw|Anti-infection Compound Library molecular weight|Anti-infection Compound Library datasheet|Anti-infection Compound Library supplier|Anti-infection Compound Library in vitro|Anti-infection Compound Library cell line|Anti-infection Compound Library concentration|Anti-infection Compound Library nmr|Anti-infection Compound Library in vivo|Anti-infection Compound Library clinical trial|Anti-infection Compound Library cell assay|Anti-infection Compound Library screening|Anti-infection Compound Library high throughput|buy Antiinfection Compound Library|Antiinfection Compound Library ic50|Antiinfection Compound Library price|Antiinfection Compound Library cost|Antiinfection Compound Library solubility dmso|Antiinfection Compound Library purchase|Antiinfection Compound Library manufacturer|Antiinfection Compound Library research buy|Antiinfection Compound Library order|Antiinfection Compound Library chemical structure|Antiinfection Compound Library datasheet|Antiinfection Compound Library supplier|Antiinfection Compound Library in vitro|Antiinfection Compound Library cell line|Antiinfection Compound Library concentration|Antiinfection Compound Library clinical trial|Antiinfection Compound Library cell assay|Antiinfection Compound Library screening|Antiinfection Compound Library high throughput|Anti-infection Compound high throughput screening| adaphostin showed one of the most highly upregulated genes to be HMOX1 (11.3 +/- 2.1 (SD) fold increase after 24 h), which encodes for an enzyme that protects against oxidative stress [7, 8]. This increase in HMOX1 expression was confirmed using Q-RT/PCR which also corroborated the lack of significant change in expression of the NRF2 gene (figure 1A). Moreover, a small but significant increase in the Nrf2 transcriptional target gene, NAD(P)H dehydrogenase, quinone 1 NQO1 was observed although there was no change in another Nrf2 target, the catalytic subunit of glutamate-cysteine NVP-BSK805 datasheet ligase GCLC (figure 1A). A significant increase in ROS production was observed
as early as 2 h after adaphostin treatment which is confirmation of the presence of drug-induced oxidative stress (figure 1B). Heme oxygenase 1, the protein encoded by HMOX1, was shown to be increased by adaphostin treatment (1 μM) at a later time point than HMOX1, being only slightly increased after 6 h, but highly expressed after 24 h (figure 1C). These data are consistent with the 10 μM adaphostin-induced heme oxygenase 1 expression reported in glioblastoma cell lines, which did not appear until after 8-24 h [6]. This adaphostin-induced HMOX1 upregulation in NCI-H522 cells and glioblastoma cell lines [6] is in contrast to the response of hematologic cell lines where we have previously reported the major transcriptional response involved
>10-fold induction of genes encoding for both heavy and light ferritin polypeptides (FTH and FTL) [3]. Moreover, even after treatment TCL with 10 μM adaphostin, leukemia cell lines (Jurkat, HL60 and K562) showed no increase in HMOX1 expression on the cDNA arrays after 6 h incubation (average expression (n = 3) = 1.24 +/- 0.7(SD), 1.35 +/- 0.39(SD) and 1.16 +/- 0.28(SD) respectively), compared to a 7.4 and 30.8 -fold increase in HMOX1 expression in NCI-H522 cells when measured on the same type of arrays following treatment with 1 and 4 μM adaphostin for 6 h. Evidence that ROS are an important factor in determining sensitivity of NCI-H522 to adaphostin was demonstrated by the ablation of adaphostin toxicity by the anti-oxidant, N-acetyl-cysteine in a manner similar to that shown for the leukemia cell line Jurkat (figure 2).