exposure. These genes were involved in such functions as transcription signal ling pathways, cytoskeleton regulation, apoptosis, meta bolism. As Differential Display is a semi quantitative method, the expression changes of the genes we were interested in, were checked by qPCR using hamster spe selleck chemical Calcitriol cific primers. qPCR was applied to RNAs not only from 24 hr treated cells, Inhibitors,Modulators,Libraries but also from cells treated for 5 hrs in order to study the cell response in the meantime. We particularly focused on changes of cytoskeleton related genes underlying morphological transformation in SHE cells. The objective was to explain from a mechanistic point of view the gene expression changes after DEHP exposure. To the best of our knowledge, this exercise has never been done previously.
Results Identification of DEHP responsive genes using Differential Display The Differential Display Inhibitors,Modulators,Libraries technique was used to identify genes differentially expressed in SHE cells, after 24 hrs of treatment with DEHP. An illustration of differen tially expressed fragments is given in Figure 1 which shows gels obtained after the DD protocol and high lights fragments regulated more than 2 fold by DEHP. Using 3 anchored primers and 80 arbitrary primers, 178 differentially expressed fragments were identified. Among these transcripts, 141 showed homology to known genes in the RefSeq database of Gen bank, while 37 had no homology or homology to hypothetical proteins. The sequences of the fragments obtained by DD have been deposited in the Genbank dbEST database.
These 141 fragments corre sponded to 122 genes that are listed in table 1 with their accession numbers and the tblastx expected. These genes were classed according to 8 biological functions with reference to the GO process database. These func tions included signal transduction and Inhibitors,Modulators,Libraries transcription, cytoskeleton regulation, xenobiotic metabolism, apoptosis, lipidogenesis, protein conformation or trans port and cell cycle. The regulation Inhibitors,Modulators,Libraries of the cytoskeleton was one of the most impacted pathways. Indeed, 21 genes involved in this function were differentially expressed after DEHP exposure. Ten genes were up regulated, and 11 were down regulated. Transcription and signal transduction is another biolo gical process targeted by DEHP treatment. We found 22 up regulated genes, among which 3 were up regulated more than 10 fold.
Heat shock response related genes and the genes involved in promoter methylation were up regulated. On the other hand, 7 genes were down regulated. Xenobiotic metabolism Carfilzomib genes such as cytochromes and glutathione S transferases were also found to be dif ferentially expressed, indicating a mobilization of cellular defence and detoxication selleck compound systems. An up regulation of cyp1b1 and cyp2e1 was registered, whereas cyp2f2 was found to be down regulated. Concerning GST, the Pi family was over expressed while the Theta and Mu families were down regulated. Differential Display results confirmed the down regu lation of c myc and showed down regula