Among them, WRKY46 transcripts showed the highest http://www.selleckchem.com/products/Cyclopamine.html induced expression after stress treatment. Compared to the untreated control, WRKY46 transcripts accumulated more quickly 4 h to 10 h after drought treatment, with the highest expression (40-fold) at 8 h after treatment. WRKY46 transcripts also accumulated quickly, at 2 h to 12 h after salt treatment, with the highest expression (70-fold) at 4 h, in comparison with the untreated control. This result suggests that WRKY46 plays important roles in the regulation of cotton abiotic stresses such as drought and salt stress. Furthermore, the expression of six WRKY genes, including WRKY59 in group I, WRKY24 and WRKY40 in
group IIa, WRKY80 in group IIb, WRKY93 in group IIe, and WRKY64 in group III, was simultaneously induced by the three stressors (drought, salt, and V. dahliae inoculation), suggesting that these WRKY genes function in the regulation of plant stress responses. Cotton, in the genus Gossypium, is the world’s most important fiber crop plant. WRKY proteins are members of a transcription factor family in higher plants that play diverse roles in plant responses to various physiological processes. In this study, based on sequence comparison and phylogenetic and structural analysis, we classified WRKY transcription factors in Gossypium into three groups (groups I, II, III), and group II genes were further classified into five subgroups (group IIa–e). Phylogenetic
analysis showed that genes in group IIa and group IIb are closely related and that group IId genes RO4929097 solubility dmso are clustered with group IIe. These results support the classifications of the three subgroups, group IIa + group Adenosine IIb, group IIc, and group IId + group IIe in group II [6] and [45]. Genes in group IIc shared more variations (80%) than genes in other WRKY groups, suggesting that WRKY genes in group IIc are more active and variable than genes in other group II subgroups. Amplification of the WRKY gene family is also related to species evolution. Zhang et al. [6] reported that numerous duplications and diversifications of WRKY genes, particularly
group III genes, have occurred since the divergence of monocots and dicots. In comparison to the 12 members of group III in G. raimondii, there are 14 and 36 group III genes in Arabidopsis and rice, respectively. These are important differences in the number of WRKY genes in dicots versus monocots. Genome-wide analysis of the WRKY gene family showed that genome duplication contributed to the accumulation of WRKY members. The previous studies reported that there were 72 WRKY family members in Arabidopsis [4], 104 members in P. trichocarpa [27], and 57 members in Vitis vinifera (http://www.phytozome.net/). In this study, we identified 120 members of the WRKY gene family in G. raimondii. The genome size of Arabidopsis is 125 Mb [46], whereas the genome sizes of P. trichocarpa, V. vinifera, and G. raimondii are 480.0, 487.0, and 737.