7; P smaller than 0.001). Multivariate analysis identified as recurrence-predicting factors anaplastic histotype (hazard ratio = 2.9; P = 0.003) and postoperative radiotherapy (hazard ratio = 4.5; P smaller than 0.001). CONCLUSIONS: The addition of adjuvant radiotherapy to surgery for atypical and anaplastic meningiomas

resulted in a clinically meaningful and statistically significant survival benefit.”
“The myelin and lymphocyte protein (MAL) is a raft-associated Selleck Buparlisib membrane protein predominantly expressed by oligodendrocytes and Schwann cells. Here we show that MAL regulates myelination in the peripheral nervous system. In mice overexpressing MAL, myelination was retarded and fibers were hypomyelinated, whereas myelination in MAL knockout mice was accelerated. This was not due to impaired Schwann cell proliferation, differentiation or axonal sorting. We found that the expression level of p75 neurotrophin receptor mRNA and protein was EPZ5676 mw strongly reduced in developing sciatic nerves in MAL-overexpressing mice. This reduction is well correlated with the observed alterations in myelination initiation, speed of myelination and alterations in Remak bundle development. Our results suggest a functional role for MAL in peripheral myelination by influencing the expression of membrane components that mediate axon-glia

interaction during ensheathment and myelin wrapping.”
“Aims:\n\nTo develop a specific, fast and simple molecular method useful to detect the entomopathogenic bacterium Pseudomonas entomophila.\n\nMethods and Results:\n\nThe use of bioinformatics tools allowed the identification of unique genes buy CH5424802 present in P. entomophila genome. Using such genes, we designed primers aiming to detect specifically P. entomophila by PCR. Furthermore, a pair of primers specifically designed

to amplify the 16S rRNA gene in Pseudomonas species was used. Primer specificity was checked using environmental pseudomonad and nonpseudomonad species. A 618 -bp fragment was amplified only in Pseudomonas using the 16S rDNA primers. Primers (PSEEN1497) designed to detect P. entomophila amplified a 570 -bp fragment only in P. entomophila. A duplex PCR was developed combining 16S rDNA and PSEEN1497 primers that allowed the detection of P. entomophila present in experimentally infected Drosophila melanogaster.\n\nConclusions:\n\nWe developed a molecular method useful to detect P. entomophila present in bacterial cultures or directly from infected insects.\n\nSignificance of the Study:\n\nTo the best of our knowledge, this is the first molecular method aiming to detect P. entomophila in environmental samples. The use of our method will facilitate studies related to ecology and insect host range of this entomopathogenic bacterium.