: 55°C; amplicon length:

500 bp Construction of the fusion TSA HDAC price protein stm0551-TOPO-F CACCATGGTGGCACAGGGTATTTTGTTAA Annealing Temp.: 50°C; amplicon length: 316 bp stm0551-TOPO-R ATATATATCTGGTAATATGGCTGG   fimY-TOPO-F CACCATGCGCAGCGTACCACGCAG Annealing Temp.: 50°C; amplicon length: 727 bp fimY-TOPO-R AAAAATGTCGTGGAAAGTAACGT   E49A-TOPO-F ATCGGCTATGCGGTCCTGACGCAACTTCCG Mutation site (underlined) E49A-TOPO-R CGGAAGTTGCGTCAGGACCGCATAGCCGAT Mutation site (underlined) RT-PCR analysis fimA-RT-F ACTATTGCGAGTCTGATGTTTG   fimA-RT-R CGTATTTCATGATAAAGGTGGC   fimZ-RT-F ATTCGTGTGATTTGGCGT Navitoclax datasheet   fimZ-RT-R ACTTATCCTGTTGACCTT   fimY-RT-F GAGTTACTGAACCAACAGCT   fimY-RT-R GCCGGTAAACTACACGATGA   fimW-RT-F AAAGTGAAAGTAAAGCGG   fimW-RT-R AAGAGATAGATAATGCCCG   stm0551-RT-F GCCATAAATAACCTTGTTCC   stm0551-RT-R CATTCATATCTCAACAGCGA

  16 s-F TTCCTCCAGATCTCTACGCA   16 s-R GTGGCTAATACCGCATAACG   Table 3 Phenotypic expression of type 1 fimbriae in  S  . Typhimurium Strain Plasmid transformed Phenotypic expression of type-1 fimbriae a     agar broth LB5010 none – ++ Δstm0551 none + ++ Δstm0551 pSTM0551 – - Δstm0551 pSTM0551E49A + ++ Δstm0551 pACYC184 + ++ a Phenotypic expression of type-1 fimbriae was determined using a mannose-sensitive yeast agglutination test and guinea pig erythrocyte hemagglutination test Figure 3 Phenotypic expression of type 1 fimbriae in  S  . Typhimurium analyzed by yeast agglutination test. S. Typhimurium LB5010 prepared from broth medium Salubrinal research buy exhibited isometheptene positive agglutination phenotype, while those prepared from agar medium showed homogenous appearance on the glass slide. Δstm0551 strain, prepared from either agar or broth medium, both demonstrated agglutination. Transforming pSTM0551 into Δstm0551 inhibited agglutination. The transformants

possessing either pSTM0551E49A or pACYC184 cloning vector exhibited the same agglutination phenotype as Δstm0551 strain. Electron microscopy S. Typhimurium LB5010 prepared in static LB broth culture demonstrated fimbrial appendages on the outermembrane of the cell (Figure 4, panel A). On the contrary, S. Typhimurium LB5010 grown on agar medium did not produce type1 fimbriae (Figure 4, panel B). The S. Typhimurium Δstm0551 strain prepared from static broth medium (Figure 4, panel C) or agar (Figure 4, panel D) produced fimbrial structures. Figure 4 Observation of  S  . Typhimurium LB5010 and the  S  . Typhimurium Δ  stm0551  strain by electron microscopy. Panel A: S. Typhimurium LB5010 obtained following growth under static LB broth conditions at 37°C for 48 h produced type 1 fimbrial appendages (40,000 ×). Panel B: No fimbrial structures were observed on the S. Typhimurium LB5010 grown on LB agar at 37°C for 18 hr (30,000 ×). Panel C: S.