Infect and Immun 2006,74(5):3016–3020.CrossRef 15. Pal U, Wang P, Bao F, Yang X, Samanta S, Schoen R, Wormser GP, Schwartz I, Fikrig E: Borrelia burgdorferi basic membrane proteins A and B participate in the genesis of Lyme arthritis. J Exp Med 2008,205(1):133–141.CrossRefPubMed 16. Jewett MW, Byram R, Bestor A, Tilly K, Lawrence K, Burtnick

MN, Gherardini F, Rosa PA: Genetic basis for retention of a critical virulence plasmid of Borrelia burgdorferi. Mol Microbiol 2007,66(4):975–990.CrossRefPubMed 17. Morrison TB, Ma Y, Weis JH, Weis JJ: Rapid and BAY 11-7082 sensitive quantification of Borrelia burgdorferi -infected mouse tissues by continuous fluorescent monitoring of PCR. J Clin Microbiol 1999,37(4):987–992.PubMed 18. Lederer S, Brenner eFT508 research buy C, Stehle T, Gern L, Wallich R, Simon MM: Quantitative analysis of Borrelia burgdorferi gene expression in naturally (tick) infected mouse strains. Med Microbiol Immunol 2005,194(1–2):81–90.CrossRefPubMed 19. Courtney JW, Massung RF: Multiplex Taqman PCR assay for rapid detection of Anaplasma phagocytophila and Borrelia burgdorferi. Ann N Y Acad Sci 2003, 990:369–370.CrossRefPubMed 20. Courtney JW, Kostelnik LM, Zeidner NS, Massung RF: Multiplex real-time PCR for detection of Anaplasma phagocytophilum and Borrelia burgdorferi. J Clin Microbiol

2004,42(7):3164–3168.CrossRefPubMed 21. Ivacic L, Reed KD, Mitchell PD, Ghebranious N: A LightCycler TaqMan assay for detection of Borrelia burgdorferi 3-mercaptopyruvate sulfurtransferase sensu lato in clinical samples. Diagn Microbiol Infect Dis 2007,57(2):137–143.CrossRefPubMed 22. Schwaiger M, Peter O, Cassinotti

P: Routine diagnosis of Borrelia burgdorferi (sensu ZD1839 mw lato) infections using a real-time PCR assay. Clin Microbiol Infect 2001,7(9):461–469.CrossRefPubMed 23. Jewett MW, Lawrence K, Bestor AC, Tilly K, Grimm D, Shaw P, Van Raden M, Gherardini F, Rosa PA: The critical role of the linear plasmid lp36 in the infectious cycle of Borrelia burgdorferi. Mol Microbiol 2007,64(5):1358–1374.CrossRefPubMed 24. Zeidner NS, Schneider BS, Dolan MC, Piesman J: An analysis of spirochete load, strain, and pathology in a model of tick-transmitted Lyme borreliosis. Vector Borne Zoonotic Dis 2001,1(1):35–44.CrossRefPubMed 25. Zeidner NS, Schneider BS, Nuncio MS, Gern L, Piesman J: Coinoculation of Borrelia spp. with tick salivary gland lysate enhances spirochete load in mice and is tick species-specific. J Parasitol 2002,88(6):1276–1278.PubMed 26. Londono D, Bai Y, Zuckert WR, Gelderblom H, Cadavid D: Cardiac apoptosis in severe relapsing fever borreliosis. Infect Immun 2005,73(11):7669–7676.CrossRefPubMed 27. Wang L, Blasic JR Jr, Holden MJ, Pires R: Sensitivity comparison of real-time PCR probe designs on a model DNA plasmid. Anal Biochem 2005,344(2):257–265.CrossRefPubMed 28. Tyagi S, Kramer FR: Molecular beacons: probes that fluoresce upon hybridization. Nat Biotechnol 1996,14(3):303–308.CrossRefPubMed 29.